A - Brucella Brucellae:
Gram-negative bacilli, are not Abuaga and non-animated.
Farm properties:
1 - Air or air, or the choice hectic air (you must secure the amount of not less than 10% of carbon dioxide and when planted).
2 - grow on the roots of a private (Kmenbt liver extract agar), and the colonies appear to be convex smooth.They ferment glucose only.
Laboratory Diagnosis:
1 - blood cultures.
2 - test Wright.
3 - test Albroocelin.
4 - Test Alawbsonin phagocytosis.
B - Alaarsenia Yarsinia:
Small Gram-negative bacilli most important types:
1 - Aarsenia plague Yarsinia pestis:
Gram-negative bacilli is small, and have a characteristiccoloration polar (edges takes a darker color from the middlewhen coloring Bozark Almtelin), non-animated and non-Mtboghand sometimes its portfolio, has a generator and a generatoragainst physical against the capsular.
Farm properties:
Almnapt grow on normal, but a distinct form colonies are small, transparent and does not analyze the coarse blood.
Laboratory Diagnosis:
1 - direct examination Babbg Bozark Almtelin (see property polarcoloration).
2 - planting on Almnapt normal.
3 - injection in test animals.
4 - serological tests: by scintillation (sparkle) immune system.
Treatment: Balttraseklin, or streptomycin, or chloramphenicol.
2 - Aarsenia Pseudotuberculosis Yarsinia pseudotuberculosis:
Gram-negative bacilli are small, polar coloration moving in class22.
3 - Aarsenia intestinal sepsis Yarsinia enterocolitica:
The same qualities and previous address Balttraseklin.
C - Lafransicela Francisella:
Gram-negative bacilli, the most important types:
Lafransicela tubaremia F. tularensis:
Gram-negative bacilli are small, exhibit an Baltleuen polarcoloration.
Farm properties:
Need to contain the roots of private egg yolks and accounts forthe amount of carbon dioxide and central air and give the colonya minute paper.
Laboratory Diagnosis:
1 - direct examination: do not give good results.
2 - Culture: the matrix contains egg yolk, blood, and after securinggas carbon dioxide and cuddling after 3-5 days we get thecolonies of thin transparent and accurate.
3 - Serological tests:
A - ELISA (indirect method) and tested the fluorinationBafilorucen.
B - skin reaction.
Laboratory test for the diagnosis of parasitic disease
Laboratory of medical parasites
The laboratory diagnosis of parasitic diseases of the flour is an important aspect in order to go to the correct methods of treatment.
Laboratory test methods: -
1. direct examination
2.indirect examination
First direct examination: -
Is one of the most important laboratory tests for being the basis ofthe initial diagnosis of the parasite and view it under a microscope.
direct stool examination: -
The tread should be treated with the same stool as a source ofinfection because it contains microorganisms, viruses, bacteria,fungi satisfactory.
Can be divided into direct examination of the feces of two parts: -
macroscopic examination
Characteristics of the stool is seen if the external liquid, solid,semi-solid, Alon, smell, blood, mucus resulting from the kit in theevent of injury.
microscopic examination
Is through the use of a single solution to facilitate the detectionand microscopy of these solutions: -
1. Physiological saline solution normal salin
2. Diluted tincture of iodine solution, iodine solution stain the nucleus of dying parasite
3. Hematoxykin hematoxylin dye used to illustrate the features ofthe parasites, especially the first.
Laboratory testing by focus constration method
There are several ways in which the concentration is stool in order to watch the parasite and the stages are many and the preparation of microscopically small area.
Resort to these methods when the test is negative but symptomspersist.
1. Flotation (flotation) flotation: -
Using saturated solutions of sugars or salts
But the solution of zinc sulfate (zinc) zinc suiphate flotation densityquality (1.18) is the best solution for the eggs of hands and bags.
How it works: take a saturated solution in a test tube and add 1 g of Albrzimzj well and then add to it a further quantity of the samesolution until the test tube is filled with half an hour left to stick tothe eggs that float on the surface of the solution cover and thenexamined under a microscope.
2. Sedimentation sedimentation: -
Way include the work of sedimentation centrifuges to speed up the deposition of eggs and bags.
Direct examination of blood direct blood examination: -
Depends on the films (flakes of blood) blood film
Way of working by taking the second drop the second or thirdfinger of the left hand.
Types of chips blood blood film: -
1.thine blood film
Nhoudrha by placing a drop of blood on a clean slide and published a slender slice along the slot and then put proven bymethyl alcohol.
2.thick blood film
Put two drops to three drops of blood in the center of the slideand one of the corners Nbstha another slide in the form of a radius of 1 cm and leave it dry at room temperature.
Chips of the most important blood stains blood film: -
1. Hman dye to Leishman stain.
2. Jmiza dye Giemsa stain.
3. Dye Field Field stain solution consists of a double blue solutionEocene Eosine
Second Alvhoud indirect indirect blood examination:
This is done after the test diagnosis is difficult to direct the parasite to the most important laboratory
1. Test immune serum Immunoserological test depends on the investigation of Antibodies.
2.Complement fixation test.
3.Haemgglutination test.
4.Immunofluorecnt test.
5.Elisa assay.
Diagnosis by agriculture Culture: -
Used in pathological cases, the few and for ease of detection forthe parasite in check Alambashrmen the most importantagricultural circles (NNN) was named after the Mkchwiha Novy), Mac Nealan, Nicolle) and add them blood and penicillin.
Used to diagnose Alchammanyh, trypanosoma.
التسميات
Analyses