First, the diagnosis depends on the history of the human disease, occupation, exposure to animals aborted, then the image of illness, bacterial isolation, and serological tests, and notes the lack of sampling during treatment with antibiotics, but before or after the meeting.
Second, to isolate Brucella in human samples are taken from the urine, feces, blood, lymph nodes, liver, spleen, bone marrow, semen, vaginal secretions, preferably taking a blood sample whilethe role of high fever, and to isolate the animals taken samplesfrom the stomach of the fetus or vaginal mucus during 3-10 weeks after abortion or childbirth, and sometimes in terms of milksecreted intermittently for life. And is implanted on the roots ofthese samples bacteriological private.
Third: Serological tests:
We depend on the detection of antibodies of both types ofanti-IgG, IgH.
1 - test assembly in vitro: Tube agglutination test In this test wereduce the serum from 1:10 to 1: 1: 1280 or more and testedagainst the standard antigen Standard antigen of the World Health Organization. The antibodies of the IgM type is the one that interacts with the test assembly in vitro is very sensitive tothis test. Show IgM in the serum after a person with 7-14 days ofonset of symptoms, and therefore used in the diagnosis of the disease in the acute phase and recorded the results as follows:
+ + + + When this happens 100% collected and deposited in fullAlonpoah.
+ + + When it happens 75% collected and deposited in fullAlonpoah.
+ + When this happens 50% collected and deposited in fullAlonpoah.
+ When this happens 25% collected and deposited in fullAlonpoah.
2 - Test the installation supplementing "CFT" Complement Fixation Test. The antibodies of the IgG class interact with the testCFT and these antibodies appear shortly in the blood of humans and animals infected by the IgM as it lasts as long as the infectionand therefore used this test CFT coupled objects immune IgM in the detection of acute, subacute, and chronic on the contrary of thetubular test assembly coupled to IgM in the serum of less than or perhaps disappear as soon as the concentration of infection in the body and therefore the latter is used in the detection of acute infection and only the absence of such antibodies does not meanthe exclusion of infection Balbrossela.
- When reading a standard positive test results in humans is 1 / 160 or more.
- When reading a standard positive serological test results in animals is 1 / 40 and over, and the 1 / 20 shall be deemed tosuspicion, and requires re-testing after 2-4 weeks and if the back of suspicion standard 1 / 20 is positive, and to kill the animal.
3 - Experimental infection in experimental animals: using guinea pigs and vulnerability of one sex where the material (blood, milk, and secretions) in the intraperitoneal or under the skin using aphysiological salt solution diluted, then the organism is grown from the spleen, liver, lymph nodes and blood.
4 - Tests of the sensitivity of the skin: Brucellin Test used to diagnose the disease in infected animals and in humans afterinjection into the skin reaction occurs locally at the injection siteskin after 24-72 hours of injection, but nothing happens in animalsor persons of others injured.
التسميات
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